Joseph: Journal of Science and Pharmacy

UJI ANTIOKSIDAN DARI FORMULASI SEDIAAN FACE MIST EKSTRAK DAUN JAMBU AIR (Syzygium samarangense)

Katharina Anrina — 2023-12-20

Background :Antioxidants are compounds that can neutralize free radicals. One of the most frequently used antioxidant cosmetic preparations is face mist. Purpose : To make face mist preparations of water guava leaf extract (Syzygium samarangense) with good physical quality and IC₅₀ value. Method : Water guava leaf extract (Syzygium samarangense) was macerated with 70% ethanol solvent and tested for water content, drying shrinkage, ethanol free, and chemical compound content. Next, evaluation of the preparation was carried out and antioxidant test were carried out on the face mist of water guava leaves (Syzygium samarangense) with concentrations of extract FI 3 grams, FII 5 grams, and FIII 7 grams using the UV-VIS spectrophotometric DPPH (1,1-diphenyl-2-picrylhydrazyl) method at wavelength of 517 nm with ratio of vitamin C. Result : This research shows that water guava leaf extract (Syzygium samarangense) can be used as a good face mist preparation and contains flavonoids, saponin, tannin, and triterpenoid which act as antioxidant. On the face mist preparation of guava leaf extract (Syzygium samarangense) produced good physical quality and the IC₅₀ value at FI was 24,54 ppm, FII was 17,61 ppm, and FIII was 8,86 ppm, so that face mist preparation had the best antioxidant content is an FIII preparation with an IC₅₀value of 8,86 ppm. Conclusion : Water guava leaf extract (Syzygium samarangense) can be made into a face mist with good physical quality and produces the best antioxidant antivity in FIII preparations with an IC₅₀ value of 8,86 ppm.

Keyword: Water guava leaf, Face mist, Antioxidant

UJI EFEKTIVITAS ANTIBAKTERI EKSTRAK ETANOL 70% DAUN BELIMBING MANIS (Averrhoa carambola L.) DARI DESA TARUB TERHADAP BAKTERI Staphylococcus aureus

Dhanisa Dwi Nugrahanti — 2023-12-20

Background: Acne or acne vulgaris is ranked as the most common disease in the world, with a prevalence of 9.4% of the world's population. Staphylococcus aureus bacteria has a percentage of 79% in causing acne. The content of secondary metabolites in plants can act as an antibacterial with a strong mechanism of action. One of them is starfruit leaves (Averrhoa carambola L.) which contain antibacterial properties such as alkaloids, flavonoids, tannins. There are many populations of this plant in Tarub Village, Tawangharjo District, Grobogan Regency

Purpose: To determine the antibacterial effectiveness of 70% ethanol extract of sweet starfruit leaves (Averrhoa carambola L.) obtained in Tarub village against Staphylococcus aureus bacteria

Method: Leaves of starfruit (Averrhoa carambola L.) were extracted by maceration method with 70% ethanol solvent and antibacterial test by paperdisk method with concentration variances of 60%, 75% 90%.

Results: Phytochemical screening of starfruit leaf extract (Averrhoa carambola L.) contains alkaloids, flavonoids, tannins, terpenoids and saponins. Antibacterial test of sweet starfruit leaf extract produced the largest inhibition zone diameter of 14.29 mm at a concentration of 60%.

Conclusion: Leaf extract of sweet star fruit effectively inhibits the growth of Staphylococcus aureus bacteria

Keyword: Sweet Carambola, antibacterial effectiveness.

ANTIBACTERIAL ACTIVITY TESTING OF COLLASE LEAF EXTRACT (Centella asiatica L.) AGAINST Propionibacterium acnes BACTERIA

Bentar Jatmiko — 2023-12-20

Background: Propionibacterium acnes or P.acnes is a normal skin flora bacteria, but can be the cause of opportunistic infections in the form of acne. Generally, acne is treated using antibacterials aimed at reducing the colonization of bacteria. The centtella asiatica plant (Centtella asiatica) is a plant of one genus as Centella. Research shows that leaf ethanol extract (Centtella asiatica) has antibacterial activity.

Purpose: This study aims to determine the antibattery activity of Centella asiatica leaf extract against Propionibacterium acnes and adequate concentration in inhibiting the growth of Propionibacterium acnes.

Method: Centella asiatica leaves (Centella asiatica) were extracted by maceration method using 70% ethanol solvent. The dieprolwh extract was carried out phytochemical tests and antibacterial activity tests with the well method against Propionibacterium acnes. Clindamycin positive control 20μg, DMSO negative control 10%.

Results: Phytochemical test of ethanol extract of centella asiatica leaves containing:

UJI AKTIVITAS SALEP EKSTRAK DAUN JAMBU AIR (Syzygium semarangense) DENGAN BASIS HIDROKARBON TERHADAP PENYEMBUHAN LUKA SAYAT PADA KELINCI

Rismawati Suci Safitri — 2023-12-20

Background: Water guava leaves contain chemical compounds in the form of alkaloids, flavonoids, saponins and tannins which are effective in healing wounds. Water guava leaves are used in several countries, including Indonesia, where they are used as a traditional medicine for healing wounds.

Objective: To determine the hydrocarbon ointment base of water guava leaves (Syzygium semarangense) which can provide activity in healing cuts in rabbits.

Method: Water guava leaf extract was obtained using the meseration method, then thickened and carried out a phytochemical screening test and thin layer chromatography to determine the compound content of water guava leaf extract which functions for healing cut wounds. Then it is formulated in the form of an ointment and the physical quality of the ointment is tested including organoleptic test, homogeneity, pH test, spreadability test, adhesiveness test, viscosity test, and ointment type test. Making an ointment formulation using water guava leaf extract using 3 (three) test animals with 5 (five) treatment groups, namely water guava leaf extract ointment with 10%, 15% and 20% hydrocarbon base, negative control with ointment base, control positive for povidone iodine 10%. All rabbits were injured with a wound length of 2 cm with a depth of ± 0.2 cm. The wound was smeared 3 times a day with the tested ointment. Wound observations were carried out every day (days 0 to 14). Data analysis was carried out using One-Way ANOVA.

Results: The results of phytochemical screening tests and thin layer chromatography show that water guava leaves contain alkaloids, flavonoids, tannins and saponins. The formulation of water guava leaf extract ointment with a hydrocarbon base meets the physical quality test requirements for ointments such as organoleptic tests, homogeneity, pH, spreadability, stickiness, viscosity and the type of ointment produced is A/M, except for the spreadability at a concentration of 20% due to the addition The higher concentration of cera alba and vaseline causes the spreadability of the ointment to become lower. The wound narrows, forms a scab and closes the wound. The statistical test results had a significant effect on wound healing in rabbits, namely (p<0.05).

Conclusion: Providing an ointment with ethanol extract of water guava leaves (Syzygium semarangense) with a hydrocarbon base at a concentration of 10% provides the most optimal effect on healing cuts in rabbits.

Determinan Health- Related Quality of Life Pasien Terapi Hemodialysis dengan Instrument EQ5D-5L

estuningtyas ayu hapsari — 2023-12-20

Background: Chronic kidney disease is caused by an increase in serum creatinine values of 20% in the kidneys. One of the management therapies for chronic kidney disease is hemodialysis therapy. The EQ5D-5L instrument is an instrument that can be used to measure the quality of chronic kidney patients. The aim of the study was to determine the description of patients with hemodialysis therapy and determine the response of patients to hemodialysis therapy with EQ5D-5L. Method: The research method is quantitative descriptive. Data collection used the EQ5D-5L instrument with the inclusion criteria of patients diagnosed with chronic kidney disease in patients aged more than 19 years. Sampling using the accidental sampling method was 100 respondents. Data analysis uses univariate analysis determinants. Results: The results of the study showed that patients with the highest range were aged 45-64 with a percentage of 67%, the proportion of male had the highest value with a percentage of 63%, the highest CCI value was in the range 3-4 with a percentage of 44% and the most therapy implementation was less than 1 year of therapy with a percentage of 59%. The utility measurement results showed that the EQ5D-5L average value was 0.595 and the VAS average value was 80, for the EQ5D-5L SD value was 0.225 and the VAS SD value was 6.464, with the EQ5D-5L median value being 0.624 and the VAS median value being 80. The most dominant response was pain at 75% and anxiety at 62%. Conclusion: Conclusion. The results of the study showed that the age of hemolysis therapy was highest in men with an average EQ5D-5L utility value of 0.595 and an average VAS value of 80. The most dominant response was pain at 75% and anxiety at 62%.