Joseph: Journal of Science and Pharmacy

UJI AKTIVITAS ANTIBAKTERI EKSTRAK DAUN PEGAGAN (Centella asiatica (L.) Urban) TERHADAP BAKTERI Staphylococcus epidermidis

Miki Rofi'atun Masriah — 2024-12-20

Background: Acne is a skin problems characterized by the appearance of papules, pustules, blackheads, and nodules. Acne can be exacerbated by the bacteria Staphylococcus epidermidis which infects inflamed skin tissue. Antibiotic can be used to treat infections, but they can cause side effects. Therefore it is necessary to do alternative medicine using natural ingredients. Pegagan (Centella asiatica (L.) Urban) leaves contain active compouds such as alkaloids, triterpenoids, saponins, flavonoids, and tannins which are antibacterial because they can damage bacterial cell walls. Purpose: The aim of this research was to determine the antibacterial activity of pegagan (Centella asiatica (L.) Urban) leaf extract against Staphylococcus epidermidis bacteria. Method: This type of research is an experimental study with the disc diffusion method. Pegagan (Centella asiatica (L.) Urban) leaves were extracted by maceration method using 70% ethanol as solvent. Pegagan (Centella asiatica (L.) Urban) leaves extract at made contrations of 60%, 80%, 100%. Results: The results showed that pegagan (Centella asiatica (L.) Urban) leaf extract with concentrations of 60%, 80% and 100% had an average zone of inhibition of 18,39 mm, 21,24 mm and 22,61 mm. Conclusion: Pegagan (Centella asiatica (L.) Urban) leaf extract has antibacterial activity against Staphylococcus epidermidis bacteria.

UJI AKTIVITAS ANTIBAKTERI SEDIAAN FACE TONER EKSTRAK ETANOL 70% DAUN SALAM (Syzygium polyanthum) TERHADAP BAKTERI Staphylococcus aureus

Sony Hartono — 2024-12-20

Background: Salam leaves contain several chemical compounds including alkaloids, flavonoids, saponins, triterpenoids and tannins which are active compounds that are effective as drugs that can cure infectious diseases caused by bacteria. Purpose: The purpose of this study was to determine the antibacterial activity of bay leaf extract face toner preparations against Staphylococcus aureus bacteria. Method: Bay leaves are extracted with 70% ethanol by maceration. The ethanol extract of bay leaves is formulated into a facial toner preparation and then tested for antibacterial activity using the disc diffusion method or the Kirby Bauer method. Results: The results of the research show that the face toner preparation with 70% ethanol extract of salam (Syzygium polyanthum) contains alkaloid compounds, flavonoids, saponins, triterpenoids and tannins. Ethanol extract of salam leaf has antibacterial activity against Staphylococcus aureus with the inhibition zone diameter of F1 of 10.28 mm, F2 of 11.24 mm, and F3 of 12.24 mm. Conclusion: Facial toner preparation of bay leaf extract (Syzygium polyanthum) provides the strongest antibacterial activity in F3 against the growth of Staphylococcus aureus bacteria with an inhibitory zone diameter of 12.24 mm.

UJI STABILITAS FISIK SEDIAAN SERUM WAJAH EKSTRAK DAUN BELIMBING MANIS (Averrhoa carambola L.) DENGAN VARIASI KONSENTRASI PROPILEN GLIKOL SEBAGAI HUMEKTAN

Devi Kurnia Saputri — 2024-12-20

Background: Sweet star fruit leaf (Averrhoa carambola L.) is a plant that contains antibacterial compounds such as alkaloids, flavonoids, saponins, tannins and triterpenoids that are used as acne therapy. Several product dosage forms have been developed for serum, one of which is in form slightly viscous. Humectants serve to improve the stability of the material over long period of time. Purpose: This research was conducted to find out at what oncentration propylene glycol as a humectant meets the physical stability test of a good serum preparations. Method: Sweet star fruit leaf (Averrhoa carambola L.) was extracted using the maceration method using methanol. The serum preparation consists of 3 formula with different concentrations of propylene glycol, namely formula I 15%, formula II 20% and formula III 25%. Evaluation of physical stability was carried out with 12 days in an accelerated test (Cycling test) with organoleptic testing parameters, homogeneity, pH, viscosity, adhesion and spreadability. Results: Serum preparations of weet star fruit leaf extract (Averrhoa carambola L.) in formula I, formula II and formula III have physical characteristics and stability that comply with SNI standards. Conclusion: The concentration of propylene glycol 25% in formula III shows the most stable serum stability properties compared to formula I and formula II.

UJI AKTIVITAS ANTI JAMUR SEDIAAN GEL EKSTRAK DAUN TURI PUTIH (Sesbania grandiflora L.) TERHADAP JAMUR Candida albicans

Sinta ardani Ardani — 2024-12-20

Background: One of the plants that has anti-fungal potential is the white Turi Plant (Sesbania grandriflora) white turi leaf extract (Sesbania grandiflora) which is suspected to be able to inhibit the growth of Candida albicans, one of the fungi that cause candidiasis. Purpose: This study aims to determine the antifungal activity of Sesbania grandriflora white turi leaf extract gel against the fungus Candida albicans, determine the minimum inhibitory concentration (KHM) and determine the content of secondary metabolite compounds. Method: The leaves (Sesbania grandriflora) are extracted by maceration with a 70% ethanol solvent. The extracts obtained were subjected to phytochemical screening tests. The antifungal activity test of turi leaf extract gel was carried out by the well method against Candida albicans. The positive control used was the 2% dandruff gel while the negative control used was the gel preparation without the extract. Results: White turi leaf extract gel (Sesbania grandriflora) has antifungal activity against Candida albicans. Conclusion: Based on the results of the research on the Antifungal Activity Test of Sesbania glandriflora White Turi Leaf Extract Gel Preparation against Candida albicans Fungus, it can be concluded as follows:

1. Sesbania glandriflora (L.) white turquoise leaf extract has the content of Saponins, flavonoids, and tannins

2. Sesbania glandriflora (L.) white turquoise leaf extract gel preparation has the activity of inhibiting the fungus Candida albicans

The most effective formulation of Sesbania glandriflora (L.) white turi extract gel preparation against the growth of Candida albicans fungus is Formulation 3 with a concentration of 8% of 20.65 mm, included in the very strong group.

Keyword: Antifungal, white dun turi extract gel, Candida albicans

UJI AKTIVITAS ANTIBAKTERI EKSTRAK KULIT BUAH NAGA MERAH (Hylocereus polyrhizus) TERHADAP BAKTERI Staphylococcus epidermidis

Iva Juliyanti — 2024-12-20

Background: Red dragon fruit skin has many benefits, but currently the utilization of red dragon fruit skin has not been carried out optimally so that it becomes environmental waste. One of the benefits is as an antibacterial because it contains flavonoids, alkaloids, saponins, and tannins. Purpose: To determine the potential of red dragon fruit peel extract in inhibiting bacterial growth against Staphylococcus epidermidis bacteria. Method: Red dragon fruit peel was macerated with 70% ethanol, characterized by specific parameters and non-specific parameters and tested for antibacterial activity using the disc method with concentrations of 10%, 20%, and 40%, then the results of the antibacterial data obtained were tested by one-way-Anova analysis and Post Hoc Tukey. Results: The yield results obtained were 10.66%, dark brown in color, sour smell, sticky and thick texture. Red dragon fruit peel extract contains flavonoids, alkaloids, tannins, saponins, and phenols. The extract is free from ethanol. The moisture content of the powder was found to be 9.85±0.057. Specific gravity 0.956±0.006. Bacterial test results obtained 10% concentration 4.1mm, 20% concentration 8.1mm, 40% concentration 16mm, positive control 31.2mm. The results of data analysis are significant at 0.000, and each concentration has a significant difference. Conclusion: Red dragon fruit peel extract has antibacterial activity against Staphylococcus epidermidis.

Keyword: Antibacterial, red dragon fruit skin extract, Staphylococcus epidermidis.